Cartoon Characters and Their Human Counterparts…

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New life: Scientists create first semi-synthetic organism with ‘alien’ DNA

SCIENCE EDITOR

The researchers believe the breakthrough is the first step towards creating new microbial life-forms with novel industrial or medical properties resulting from a potentially massive expansion of genetic information.

The semi-synthetic microbe, a genetically modified E. coli bacterium, has been endowed with an extra artificial piece of DNA with an expanded genetic alphabet – instead of the usual four “letters” of the alphabet its DNA molecule has six.

The natural genetic code of all living things is based on a sequence of four bases – G, C, T, A – which form two sets of bonded pairs, G to C and T to A, that link the two strands of the DNA double helix.

The DNA of the new semi-synthetic microbe, however, has a pair of extra base pairs, denoted by X and Y, which pair up together like the other base pairs and are fully integrated into the rest of the DNA’s genetic code.

The scientists said that the semi-synthetic E. coli bacterium replicates normally and is able to pass on the new genetic information to subsequent generations. However, it was not able to use the new encoded information to produce any novel proteins – the synthetic DNA was added as an extra circular strand that did not take part in the bacterium’s normal metabolic functions.

The study, published in the journal Nature, is the first time that scientists have managed to produce a genetically modified microbe that is able to function and replicate with a different genetic code to the one that is thought to have existed ever since life first started to evolve on Earth more than 3.5 billion years ago.

“Life on earth in all its diversity is encoded by only two pairs of DNA bases, A-T and C-G, and what we’ve made is an organism that stably contains those two plus a third, unnatural pair of bases,” said Professor Floyd Romesberg of the Scripps Research Institute in La Jolla, California.

“This shows that other solutions to storing information are possible and, of course, takes us closer to an expanded-DNA biology that will have many exciting applications, from new medicines to new kinds of nanotechnology,” Professor Romesberg said.

Expanding the genetic code with an extra base pair raises the prospect of building new kinds of proteins from a much wider range of amino acids than the 20 or so that exist in nature. A new code based on six base pairs could in theory deal with more than 200 amino acids, the scientists said.

“In principle, we could encode new proteins made from new, unnatural amino acids, which would give us greater power than ever to tailor protein therapeutics and diagnostics and laboratory reagents to have desired functions,” Professor Romesberg said.

“Other applications, such as nanomaterials, are also possible,” he added.

The researchers emphasised that there is little danger of the new life-forms living outside the confines of the laboratory, as they are not able to replicate with their synthetic DNA strand unless they are continuously fed the X and Y bases – synthetic chemicals called “d5SICS” and “dNaM”, that do not exist in nature.

The bacteria also need a special protein to transport the new bases around the cell of the microbe. The transporter protein comes from algae and if it, or the X and Y bases, are lacking, the microbial cells revert back to the natural genetic code, said Denis Malyshev of the Scripps Institute.

“Our new bases can only get into the cell if we turn on the “base transporter” protein. Without this transporter or when the new bases are not provided, the cell will revert back to A, T, G, C and the d5SICS and the dNaM will disappear from the genome,” Dr Malyshev said.

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8-year-old YouTube star makes $1.3 million per year

Prepare yourselves: You’re about to become jealous of an 8-year-old. Why, you ask? Well, let’s just say it’s not because of an epic collection of Legos or Barbies.  Here is his YouTube channel.

https://www.youtube.com/channel/UCHa-hWHrTt4hqh-WiHry3Lw

Evan spends his time reviewing toys and video games. He and his family create entertaining videos that often involve some special effects and time-lapse magic and post them on YouTube.

And then there’s the fact that Evan’s videos get millions of views. His Pirate Pig Attack Game video has received almost 7 million so far.

And finally, Evan’s YouTube channel brings home an incredible $1.3 million each year, according to Business Insider.

Newsweek reports the majority of that sum is made from ad revenue. But Evan’s father, Jared, told the publication Evan doesn’t even know about all the money and his popularity online.”For the most part, Evan goes about the day like any other second-grader. He goes to school, does his homework, hangs out with his friends, attends karate class, and, of course, he has his computer time.”

“Good Morning America” had Evan on the show Tuesday morning to talk about how he got started making his videos.

Evan explained, “I watched YouTube, and I guess asked my dad if I could start a YouTube channel.”

Michael Strahan asked, “Now, what is your all-time favorite toy? That’s what I want to know.” Evan replied, “Um, LEGOS!”

We decided to see how he stacked up against some other young, self-made millionaires.

App designer Nick D’Aloisio sold his news app to Yahoo for a whopping $30 million when he was just 17 years old.

And Evan’s already surpassed 15-year-old Madison Robinson. Last year Forbes reported she earned over $1 million in sales with her Fish Flops.

Seems like Evan’s in pretty good company. And as for all that money he’s raking in? He’ll be able to spend it when he’s older. For now, it’s in investment accounts for him and his sister, who also stars in the videos.

http://www.aol.com/article/2014/09/30/8-year-old-youtube-star-makes-1-3-million-per-year/20970024/?ncid=txtlnkusaolp00000058

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Forbes – Best 100 Inspirational Quotes

I have taken the liberty to BOLD the ones I agree with completely. – Michael Bradley

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Kevin Kruse

Contributor

I write about wholehearted leadership and employee engagement.

Opinions expressed by Forbes Contributors are their own.

100 Best Quotes On Leadership

Kevin Kruse
Contributor

LEADERSHIP 5/28/2013
Top 100 Inspirational Quotes

Inspirational quotes and motivational quotes have the power to get us through a bad week, and can even give us the courage to pursue our life’s dreams. In my book, 4 Keys to Happiness and Fulfillment at Work, I share surprising research into the true triggers of workplace motivation. So in the spirit of self motivation, here are 100 inspirational quotes.

ForbesQuotes: Thoughts On The Business Of Life
1. Life isn’t about getting and having, it’s about giving and being. –Kevin Kruse

2. Whatever the mind of man can conceive and believe, it can achieve. –Napoleon Hill

3. Strive not to be a success, but rather to be of value. –Albert Einstein

4. Two roads diverged in a wood, and I—I took the one less traveled by, And that has made all the difference. –Robert Frost

5. I attribute my success to this: I never gave or took any excuse. –Florence Nightingale

6. You miss 100% of the shots you don’t take. –Wayne Gretzky

7. I’ve missed more than 9000 shots in my career. I’ve lost almost 300 games. 26 times I’ve been trusted to take the game winning shot and missed. I’ve failed over and over and over again in my life. And that is why I succeed. –Michael Jordan

8. The most difficult thing is the decision to act, the rest is merely tenacity. –Amelia Earhart

9. Every strike brings me closer to the next home run. –Babe Ruth

10. Definiteness of purpose is the starting point of all achievement. –W. Clement Stone

11. We must balance conspicuous consumption with conscious capitalism. –Kevin Kruse

12. Life is what happens to you while you’re busy making other plans. –John Lennon

13. We become what we think about. –Earl Nightingale

14.Twenty years from now you will be more disappointed by the things that you didn’t do than by the ones you did do, so throw off the bowlines, sail away from safe harbor, catch the trade winds in your sails. Explore, Dream, Discover. –Mark Twain

15.Life is 10% what happens to me and 90% of how I react to it. –Charles Swindoll

16. The most common way people give up their power is by thinking they don’t have any. –Alice Walker

17. The mind is everything. What you think you become. –Buddha

18. The best time to plant a tree was 20 years ago. The second best time is now. –Chinese Proverb

19. An unexamined life is not worth living. –Socrates

20. Eighty percent of success is showing up. –Woody Allen

21. Your time is limited, so don’t waste it living someone else’s life. –Steve Jobs

22. Winning isn’t everything, but wanting to win is. –Vince Lombardi

23. I am not a product of my circumstances. I am a product of my decisions. –Stephen Covey

24. Every child is an artist. The problem is how to remain an artist once he grows up. –Pablo Picasso

25. You can never cross the ocean until you have the courage to lose sight of the shore. –Christopher Columbus

The Forbes eBook Of Motivational Quotes
Discover the timeless advice that the world’s great thinkers, billionaires, writers and businesspeople have to offer.

26. I’ve learned that people will forget what you said, people will forget what you did, but people will never forget how you made them feel. –Maya Angelou

27. Either you run the day, or the day runs you. –Jim Rohn

28. Whether you think you can or you think you can’t, you’re right. –Henry Ford

29. The two most important days in your life are the day you are born and the day you find out why. –Mark Twain

30. Whatever you can do, or dream you can, begin it. Boldness has genius, power and magic in it. –Johann Wolfgang von Goethe

31. The best revenge is massive success. –Frank Sinatra

32. People often say that motivation doesn’t last. Well, neither does bathing. That’s why we recommend it daily. –Zig Ziglar

33. Life shrinks or expands in proportion to one’s courage. –Anais Nin

34. If you hear a voice within you say “you cannot paint,” then by all means paint and that voice will be silenced. –Vincent Van Gogh

35. There is only one way to avoid criticism: do nothing, say nothing, and be nothing. –Aristotle

36. Ask and it will be given to you; search, and you will find; knock and the door will be opened for you. –Jesus

37. The only person you are destined to become is the person you decide to be. –Ralph Waldo Emerson

38. Go confidently in the direction of your dreams. Live the life you have imagined. –Henry David Thoreau

39. When I stand before God at the end of my life, I would hope that I would not have a single bit of talent left and could say, I used everything you gave me. –Erma Bombeck

40. Few things can help an individual more than to place responsibility on him, and to let him know that you trust him. –Booker T. Washington

41. Certain things catch your eye, but pursue only those that capture the heart. – Ancient Indian Proverb

42. Believe you can and you’re halfway there. –Theodore Roosevelt

43. Everything you’ve ever wanted is on the other side of fear. –George Addair

44. We can easily forgive a child who is afraid of the dark; the real tragedy of life is when men are afraid of the light. –Plato

45. Teach thy tongue to say, “I do not know,” and thous shalt progress. –Maimonides

46. Start where you are. Use what you have. Do what you can. –Arthur Ashe

47. When I was 5 years old, my mother always told me that happiness was the key to life. When I went to school, they asked me what I wanted to be when I grew up. I wrote down ‘happy’. They told me I didn’t understand the assignment, and I told them they didn’t understand life. –John Lennon

48. Fall seven times and stand up eight. –Japanese Proverb

49. When one door of happiness closes, another opens, but often we look so long at the closed door that we do not see the one that has been opened for us. –Helen Keller

50. Everything has beauty, but not everyone can see. –Confucius

51. How wonderful it is that nobody need wait a single moment before starting to improve the world. –Anne Frank

52. When I let go of what I am, I become what I might be. –Lao Tzu

53. Life is not measured by the number of breaths we take, but by the moments that take our breath away. –Maya Angelou

54. Happiness is not something readymade. It comes from your own actions. –Dalai Lama

55. If you’re offered a seat on a rocket ship, don’t ask what seat! Just get on. –Sheryl Sandberg

56. First, have a definite, clear practical ideal; a goal, an objective. Second, have the necessary means to achieve your ends; wisdom, money, materials, and methods. Third, adjust all your means to that end. –Aristotle

57. If the wind will not serve, take to the oars. –Latin Proverb

58. You can’t fall if you don’t climb. But there’s no joy in living your whole life on the ground. –Unknown

59. We must believe that we are gifted for something, and that this thing, at whatever cost, must be attained. –Marie Curie

60. Too many of us are not living our dreams because we are living our fears. –Les Brown

61. Challenges are what make life interesting and overcoming them is what makes life meaningful. –Joshua J. Marine

62. If you want to lift yourself up, lift up someone else. –Booker T. Washington

63. I have been impressed with the urgency of doing. Knowing is not enough; we must apply. Being willing is not enough; we must do. –Leonardo da Vinci

64. Limitations live only in our minds. But if we use our imaginations, our possibilities become limitless. –Jamie Paolinetti

65. You take your life in your own hands, and what happens? A terrible thing, no one to blame. –Erica Jong

66. What’s money? A man is a success if he gets up in the morning and goes to bed at night and in between does what he wants to do. –Bob Dylan

67. I didn’t fail the test. I just found 100 ways to do it wrong. –Benjamin Franklin

68. In order to succeed, your desire for success should be greater than your fear of failure. –Bill Cosby

69. A person who never made a mistake never tried anything new. – Albert Einstein

70. The person who says it cannot be done should not interrupt the person who is doing it. –Chinese Proverb

71. There are no traffic jams along the extra mile. –Roger Staubach

72. It is never too late to be what you might have been. –George Eliot

73. You become what you believe. –Oprah Winfrey

74. I would rather die of passion than of boredom. –Vincent van Gogh

75. A truly rich man is one whose children run into his arms when his hands are empty. –Unknown

76. It is not what you do for your children, but what you have taught them to do for themselves, that will make them successful human beings. –Ann Landers

77. If you want your children to turn out well, spend twice as much time with them, and half as much money. –Abigail Van Buren

78. Build your own dreams, or someone else will hire you to build theirs. –Farrah Gray

79. The battles that count aren’t the ones for gold medals. The struggles within yourself–the invisible battles inside all of us–that’s where it’s at. –Jesse Owens

80. Education costs money. But then so does ignorance. –Sir Claus Moser

81. I have learned over the years that when one’s mind is made up, this diminishes fear. –Rosa Parks

82. It does not matter how slowly you go as long as you do not stop. –Confucius

83. If you look at what you have in life, you’ll always have more. If you look at what you don’t have in life, you’ll never have enough. –Oprah Winfrey

84. Remember that not getting what you want is sometimes a wonderful stroke of luck. –Dalai Lama

85. You can’t use up creativity. The more you use, the more you have. –Maya Angelou

86. Dream big and dare to fail. –Norman Vaughan

87. Our lives begin to end the day we become silent about things that matter. –Martin Luther King Jr.

88. Do what you can, where you are, with what you have. –Teddy Roosevelt

89. If you do what you’ve always done, you’ll get what you’ve always gotten. –Tony Robbins

90. Dreaming, after all, is a form of planning. –Gloria Steinem

91. It’s your place in the world; it’s your life. Go on and do all you can with it, and make it the life you want to live. –Mae Jemison

92. You may be disappointed if you fail, but you are doomed if you don’t try. –Beverly Sills

93. Remember no one can make you feel inferior without your consent. –Eleanor Roosevelt

94. Life is what we make it, always has been, always will be. –Grandma Moses

95. The question isn’t who is going to let me; it’s who is going to stop me. –Ayn Rand

96. When everything seems to be going against you, remember that the airplane takes off against the wind, not with it. –Henry Ford

97. It’s not the years in your life that count. It’s the life in your years. –Abraham Lincoln

98. Change your thoughts and you change your world. –Norman Vincent Peale

99. Either write something worth reading or do something worth writing. –Benjamin Franklin

100. Nothing is impossible, the word itself says, “I’m possible!” –Audrey Hepburn

101. The only way to do great work is to love what you do. –Steve Jobs

102. If you can dream it, you can achieve it. –Zig Ziglar

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Punny Literary Sentences

For those who love a punny turn of phrase…

42 phrases a lexophile would love

Lexophile

1. I wondered why the baseball was getting bigger. Then it hit me.

2. Police were called to a day care, where a three-year-old was resisting a rest.

3. Did you hear about the guy whose whole left side was cut off? He’s all right now.

4. The roundest knight at King Arthur’s round table was Sir Cumference.

5. To write with a broken pencil is pointless.

6. When fish are in schools they sometimes take debate.

7. The short fortune teller who escaped from prison was a small medium at large.

8. A thief who stole a calendar… got twelve months.

9. A thief fell and broke his leg in wet cement. He became a hardened criminal.

10. Thieves who steal corn from a garden could be charged with stalking.

11. When the smog lifts in Los Angeles , U. C. L. A.

12. The math professor went crazy with the blackboard. He did a number on it.

13. The professor discovered that his theory of earthquakes was on shaky ground.

14. The dead batteries were given out free of charge.

15. If you take a laptop computer for a run you could jog your memory.

16. A dentist and a manicurist fought tooth and nail.

17. A bicycle can’t stand alone; it is two tired.

18. A will is a dead giveaway.

19. Time flies like an arrow; fruit flies like a banana.

20. A backward poet writes inverse.

21. In a democracy it’s your vote that counts; in feudalism, it’s your Count that votes.

22. A chicken crossing the road: poultry in motion.

23. If you don’t pay your exorcist you can get repossessed.

24. With her marriage she got a new name and a dress.

25. Show me a piano falling down a mine shaft and I’ll show you A-flat miner.

26. When a clock is hungry it goes back four seconds.

27. The guy who fell onto an upholstery machine was fully recovered.

28. A grenade fell onto a kitchen floor in France and resulted in Linoleum Blownapart.

29. You are stuck with your debt if you can’t budge it.

30. Local Area Network in Australia : The LAN down under.

31. He broke into song because he couldn’t find the key.

32. A calendar’s days are numbered.

33. A boiled egg is hard to beat.

34. He had a photographic memory which was never developed.

35. A plateau is a high form of flattery.

36. Those who get too big for their britches will be exposed in the end.

37. When you’ve seen one shopping center you’ve seen a mall.

38. If you jump off a Paris bridge, you are in Seine.

39. Bakers trade bread recipes on a knead to know basis.

40. Santa’s helpers are subordinate clauses.

41. Acupuncture: a jab well done.

42. A lot of money is tainted: ‘Taint yours, and ‘taint mine.

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Cute Dogs for Your Monday Blues!

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September 29, 2014 · 2:56 pm

Let’s hope he’s lucky! Baby calf born with a perfect white number seven on his head

  • A Pennsylvania farm have named a calf Baby Ben after the Pittsburgh Steelers quarterback because the cow has a No. 7 on his head 
  • After photos of the calf were posted on the vale Wood Farms Facebook page, almost 2,000 fans liked the posting
  • Vale Wood Farms has been around since 1933 and while it processes its own milk and dairy, it doesn’t use any meat from the cows.

By ASSOCIATED PRESS and ALEXANDRA KLAUSNER

Moooove over, Ben Roethlisberger. There’s a new No. 7 in western Pennsylvania.

The folks at Vale Wood Farms in Loretto have named a calf Baby Ben after the Pittsburgh Steelers quarterback because the cow has a marking that looks like a Number 7 on its head.

The farm has posted the calf’s picture on its Facebook page, and WJAC-TV visited the farm about 70 miles east of Pittsburgh to see what all the fuss is about.

Lucky number seven: A baby calf with a marking of a number 7 on its head has been named Big Ben in honor of Pittsburgh Steelers quarterback Ben Roethlisberger who wears a seven on his jersey when he plays

Look familiar?: Baby Ben is named after Steelers quarterback Ben Roethlisberger (pictured) who wears a number seven on his jersey and is nicknamed Big Ben 

Since being posted on Facebook the photos got over 1,100 likes and over 100 comments.

Carissa Itle-Westrick, the director of business development for the family-owned food-and-grocery farm, is hoping Roethlisberger won’t mind.

She says, ‘Baby Ben is awfully cute, so hopefully Big Ben won’t mind sharing his namesake.’

The farm plans to display the calf when it opens its pumpkin patch for business next month.

Itle-Westrick told the Pittsburgh Post-Gazette that she’s seen a variety of cow patterns over the years such as a state, a continent, and once even a Nike’s swoosh.

She said that when she saw Baby Ben for the first time she immediately thought of the Steelers.

‘He turned to face me, and I said, “Wow, it’s a perfect little 7,” ‘ Ms. Itle-Westrick said. Taking the quarterback’s namesake

‘was kind of a natural for us. We’re out here in Steelers country.’

Even Ben Roethlisberger’s website Bigben7.com acknowledged the furry doppelganger in a blog post.

The post said that if you visit the farm, ‘don’t forget to say hello to Baby Ben while you’re there (who is probably gonna milk this new-found fame for all it’s worth!)’

The Post gazette reports that Baby Ben will be on display during the farm’s pumpkin patch event October 1-19.

Vale Wood Farms has been around since 1933 and while it processes its own milk and dairy, it doesn’t use any meat from the cows.

Head in the game: Even Ben Roethlisberger's website acknowledged the furry doppelganger and he wrote in his blog of visiting the farm, 'don’t forget to say hello to Baby Ben while you’re there'

Head in the game: Even Ben Roethlisberger’s website acknowledged the furry doppelganger and he wrote in his blog of visiting the farm, ‘don’t forget to say hello to Baby Ben while you’re there’

Read more: http://www.dailymail.co.uk/news/article-2771662/Adorable-baby-calf-born-NUMBER-SEVEN-head-named-Big-Ben-honor-Pittsburgh-Steelers-quarterback-Ben-Ben-Roethlisberger-wears-number-seven-jersey.html#ixzz3EaMv4Oc9
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