Tag Archives: genetic engineering

New life: Scientists create first semi-synthetic organism with ‘alien’ DNA

Wednesday 07 May 2014

The researchers believe the breakthrough is the first step towards creating new microbial life-forms with novel industrial or medical properties resulting from a potentially massive expansion of genetic information.

The semi-synthetic microbe, a genetically modified E. coli bacterium, has been endowed with an extra artificial piece of DNA with an expanded genetic alphabet – instead of the usual four “letters” of the alphabet its DNA molecule has six.

The natural genetic code of all living things is based on a sequence of four bases – G, C, T, A – which form two sets of bonded pairs, G to C and T to A, that link the two strands of the DNA double helix.

The DNA of the new semi-synthetic microbe, however, has a pair of extra base pairs, denoted by X and Y, which pair up together like the other base pairs and are fully integrated into the rest of the DNA’s genetic code.

The scientists said that the semi-synthetic E. coli bacterium replicates normally and is able to pass on the new genetic information to subsequent generations. However, it was not able to use the new encoded information to produce any novel proteins – the synthetic DNA was added as an extra circular strand that did not take part in the bacterium’s normal metabolic functions.

The study, published in the journal Nature, is the first time that scientists have managed to produce a genetically modified microbe that is able to function and replicate with a different genetic code to the one that is thought to have existed ever since life first started to evolve on Earth more than 3.5 billion years ago.

“Life on earth in all its diversity is encoded by only two pairs of DNA bases, A-T and C-G, and what we’ve made is an organism that stably contains those two plus a third, unnatural pair of bases,” said Professor Floyd Romesberg of the Scripps Research Institute in La Jolla, California.

“This shows that other solutions to storing information are possible and, of course, takes us closer to an expanded-DNA biology that will have many exciting applications, from new medicines to new kinds of nanotechnology,” Professor Romesberg said.

Expanding the genetic code with an extra base pair raises the prospect of building new kinds of proteins from a much wider range of amino acids than the 20 or so that exist in nature. A new code based on six base pairs could in theory deal with more than 200 amino acids, the scientists said.

“In principle, we could encode new proteins made from new, unnatural amino acids, which would give us greater power than ever to tailor protein therapeutics and diagnostics and laboratory reagents to have desired functions,” Professor Romesberg said.

“Other applications, such as nanomaterials, are also possible,” he added.

The researchers emphasised that there is little danger of the new life-forms living outside the confines of the laboratory, as they are not able to replicate with their synthetic DNA strand unless they are continuously fed the X and Y bases – synthetic chemicals called “d5SICS” and “dNaM”, that do not exist in nature.

The bacteria also need a special protein to transport the new bases around the cell of the microbe. The transporter protein comes from algae and if it, or the X and Y bases, are lacking, the microbial cells revert back to the natural genetic code, said Denis Malyshev of the Scripps Institute.

“Our new bases can only get into the cell if we turn on the “base transporter” protein. Without this transporter or when the new bases are not provided, the cell will revert back to A, T, G, C and the d5SICS and the dNaM will disappear from the genome,” Dr Malyshev said.

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Reminder of My Panels at LepreCon 38

LepreCon 38 starts TOMORROW, it goes Thursday through Sunday!

The event is held at the Tempe Mission Palms in Tempe, Arizona.  For a link to find out more, go to:


I will be appearing in seven panels, a book signing, and a book reading, as well as selling autographed copies of my first two books in the Dealer Room.  Hope to see you there.

My personal schedule:


LIT-Good, Bad & Ugly of Genre Fiction 1-2pm Palm E

Whether it’s made into a movie and television show, some of these genre fiction stories are good or bad for their fans. We will discuss when such stories get ugly for the general population. What’s good and bad about Twilight, True Blood, and other genre fiction works.

SCI – Laboratory went Boom 6-7pm Joshua Tree

A discussion on laboratory mistakes and how they lead to discoveries and/or spectacular failures.


 SOC-Here comes the Apocalypse 2-3pm Xavier

The Noah story and other end of days’ theories – facts or stuff for the fiction books? Why are the apocalyptic story lines so prevalent in today’s television, movies and books?

Autograph session in dealer’s room 3-4pm

 SCI-The Stem Cell Hamburger Drive-Thru 4-5pm Xavier

Stem Cell Hamburgers, would you eat them?  A discussion on the current state of genetically engineered food and animal cloning in general.

 LIT – Independent Publishing Update 5:00-6:30pm Palm F

What’s new on the horizon for the Indie authors and publishers? There is so much changing that it’s hard to keep up with the technology used to support the pros and entertain the masses.

LIT/MED – Why is Steampunk picking up steam 7-8 pm Palm E

Sherlock Holmes, Wild Wild West, The Three Musketeers have all shared a new major element in their story lines and that is adding steampunk. Why has the turn of the century historical costumes, gadgets and ideas become so popular today?


 Michael Bradley Reading 11-11:30am Boardroom

Excerpts from Two Books – The Travelers’ Club and The Ghost Ship and Twisted History

SCI/LIT – Science research for book writing 3-4pm Xavier

Questions that will be discussed: Will guns rust in space? If we use super hot water for propulsion, how will the engines hold up or work, long term? Can we recycle the space junk left behind by the Apollo teams when we live on the Moon? Can we make a multiple planetary lander today or can we even expect this in the future?

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